Renal ectopic lipid deposition in rats with early-stage diabetic nephropathy evaluated by the MR mDixon-Quant technique: association with the expression of SREBP-1 and PPARα in renal tissue.
Quant Imaging Med Surg
; 13(7): 4504-4513, 2023 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-37456311
ABSTRACT
Background:
Renal ectopic lipid deposition (ELD) plays a significant role in the development of diabetic nephropathy (DN). This study aimed to use the magnetic resonance (MR) mDixon-Quant technique to evaluate renal ELD and its association with the expression of sterol regulatory element binding protein 1 (SREBP-1) and peroxisome proliferator-activated receptor alpha (PPARα) in renal tissue.Methods:
Seventy male Sprague-Dawley (SD) rats were randomly divided into experimental (n=50) and control groups (n=20). A high-fat diet combined with low-dose streptozotocin (STZ) was administered to the experimental group to establish a type 2 diabetes mellitus (T2DM) model. The rats received renal mDixon-Quant scans and blood lipid and histopathological examinations in batches after the T2DM model was established. According to the histopathological findings, the included rats were stratified into control and early DN groups. Renal fat fraction (FF), blood lipid level, the ratio of the integrated optical density of intracellular lipid droplets and the total area of all the cells (IOD/TAC), and the expression of SREBP-1 and PPARÉ in renal tissue were analyzed.Results:
Compared to the controls, renal FF, IOD/TAC, the expression of SREBP-1 in renal tissue, and serum total cholesterol (TC), triglyceride (TG) and low-density lipoprotein (LDL) levels were higher in the early DN group, while the expression of PPARÉ in renal tissue and the high-density lipoprotein (HDL) level were lower (all P values <0.001). Renal FF gradually increased with the progression of disease [r=0.810 (95% CI 0.675-0.928), P<0.001]. Positive correlations between renal FF and each of the following TC, TG, LDL, IOD/TAC, and the expression of SREBP-1 [r=0.479 (95% CI 0.353-0.640, P=0.012), 0.576 (95% CI 0.283-0.842, P=0.002), 0.441 (95% CI 0.305-0.606, P=0.021), 0.911 (95% CI 0.809-0.964, P<0.001) and 0.800 (95% CI 0.640-0.910, P<0.001), respectively] and negative correlations between renal FF and each of the following HDL and the expression of PPARÉ [r=-0.611 (95% CI -0.809 to -0.469, P=0.001) and -0.748 (95% CI -0.886 to -0.585, P<0.001), respectively] were found.Conclusions:
Renal lipid deposition evaluated by the MR mDixon-Quant technique is associated with the blood lipid level, histological fat quantification, and the expression of SREBP-1 and PPARÉ in renal tissue. The renal FF value might serve as a biomarker for better understanding of renal lipid metabolism in early-stage DN.
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Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article