Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells.
Methods Mol Biol
; 2677: 269-280, 2023.
Article
em En
| MEDLINE
| ID: mdl-37464248
ABSTRACT
Dynamic pluripotent stem cell (PSC) states are in vitro adaptations of the pluripotency continuum in vivo. Previous studies have generated a number of PSCs with distinct properties. By modulating the FGF, TGF-ß, and WNT pathways, we have derived intermediate PSCs (FTW-PSCs) that are permissive for direct primordial germ cell-like cell (PGC-LC) induction in vitro. Here, we describe the method for derivation and maintenance of mouse and human FTW-PSCs, as well as PGC-LC induction from FTW-PSCs.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Pluripotentes
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article