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Open-3DSIM: an open-source three-dimensional structured illumination microscopy reconstruction platform.
Cao, Ruijie; Li, Yaning; Chen, Xin; Ge, Xichuan; Li, Meiqi; Guan, Meiling; Hou, Yiwei; Fu, Yunzhe; Xu, Xinzhu; Leterrier, Christophe; Jiang, Shan; Gao, Baoxiang; Xi, Peng.
Afiliação
  • Cao R; Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China.
  • Li Y; National Biomedical Imaging Center, Peking University, Beijing, China.
  • Chen X; Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China.
  • Ge X; National Biomedical Imaging Center, Peking University, Beijing, China.
  • Li M; Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China.
  • Guan M; National Biomedical Imaging Center, Peking University, Beijing, China.
  • Hou Y; Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environment Science, Hebei University, Baoding, China.
  • Fu Y; Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China.
  • Xu X; National Biomedical Imaging Center, Peking University, Beijing, China.
  • Leterrier C; Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China.
  • Jiang S; National Biomedical Imaging Center, Peking University, Beijing, China.
  • Gao B; Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China.
  • Xi P; National Biomedical Imaging Center, Peking University, Beijing, China.
Nat Methods ; 20(8): 1183-1186, 2023 08.
Article em En | MEDLINE | ID: mdl-37474809
ABSTRACT
Open-3DSIM is an open-source reconstruction platform for three-dimensional structured illumination microscopy. We demonstrate its superior performance for artifact suppression and high-fidelity reconstruction relative to other algorithms on various specimens and over a range of signal-to-noise levels. Open-3DSIM also offers the capacity to extract dipole orientation, paving a new avenue for interpreting subcellular structures in six dimensions (xyzθλt). The platform is available as MATLAB code, a Fiji plugin and an Exe application to maximize user-friendliness.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Iluminação / Microscopia Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Iluminação / Microscopia Idioma: En Ano de publicação: 2023 Tipo de documento: Article