Serum-free lentiviral vector production is compatible with medium-resident nuclease activity arising from adherent HEK293T host cells engineered with a nuclease-encoding transgene.

Ali, Sadfer; Rivera, Milena; Ward, John; Keshavarz-Moore, Eli; Mason, Chris; Nesbeth, Darren N

Ali, Sadfer; Rivera, Milena; Ward, John; Keshavarz-Moore, Eli; Mason, Chris; Nesbeth, Darren N.

Afiliação

Ali S; The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, WC1E 7JE, UK.
Rivera M; The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, WC1E 7JE, UK.
Ward J; The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, WC1E 7JE, UK.
Keshavarz-Moore E; The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, WC1E 7JE, UK.
Mason C; The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, WC1E 7JE, UK.
Nesbeth DN; The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, WC1E 7JE, UK.

Heliyon ; 9(6): e17067, 2023 Jun.

Article em En | MEDLINE | ID: mdl-37484388