Clinical evaluation of a dermatophyte RT-PCR assay and its impact on the turn-around-time: A prospective study.

Debuysschere, Cyril; Blairon, Laurent; Cupaiolo, Roberto; Beukinga, Ingrid; Tré-Hardy, Marie

Debuysschere, Cyril; Blairon, Laurent; Cupaiolo, Roberto; Beukinga, Ingrid; Tré-Hardy, Marie.

Afiliação

Debuysschere C; Department of Laboratory Medicine, Iris Hospitals South, Brussels, Belgium.
Blairon L; Department of Laboratory Medicine, Iris Hospitals South, Brussels, Belgium.
Cupaiolo R; Department of Laboratory Medicine, Iris Hospitals South, Brussels, Belgium.
Beukinga I; Department of Laboratory Medicine, Iris Hospitals South, Brussels, Belgium.
Tré-Hardy M; Department of Laboratory Medicine, Iris Hospitals South, Brussels, Belgium.

Med Mycol ; 61(8)2023 Aug 02.

Article em En | MEDLINE | ID: mdl-37505466

The aim of this study was to evaluate the clinical performance of a multiplex PCR in the identification of the causative agent of onychomycosis on nail samples, and its impact on the turnaround time, compared to our traditional laboratory methods. This new method is rapid, reliable, robust, and inexpensive.

Assuntos

Arthrodermataceae; Onicomicose; Animais; Onicomicose/diagnóstico; Onicomicose/veterinária; Arthrodermataceae/genética; Estudos Prospectivos; Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária; DNA Fúngico; Sensibilidade e Especificidade; Reação em Cadeia da Polimerase Multiplex/métodos; Reação em Cadeia da Polimerase Multiplex/veterinária; Trichophyton/genética

Palavras-chave

RT-PCR; clinical evaluation; dermatophytes; onychomycosis

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Texto completo: 1 Eixos temáticos: Pesquisa_clinica Base de dados: MEDLINE Assunto principal: Onicomicose / Arthrodermataceae Tipo de estudo: Diagnostic_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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