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Bone on-a-chip: a 3D dendritic network in a screening platform for osteocyte-targeted drugs.
Lipreri, Maria Veronica; Di Pompo, Gemma; Boanini, Elisa; Graziani, Gabriela; Sassoni, Enrico; Baldini, Nicola; Avnet, Sofia.
Afiliação
  • Lipreri MV; Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
  • Di Pompo G; Biomedical Science, Technologies, and Nanobiotecnologiy Lab, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.
  • Boanini E; Department of Chemistry 'Giacomo Ciamician', University of Bologna, Bologna, Italy.
  • Graziani G; Biomedical Science, Technologies, and Nanobiotecnologiy Lab, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.
  • Sassoni E; Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, Bologna, Italy.
  • Baldini N; Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
  • Avnet S; Biomedical Science, Technologies, and Nanobiotecnologiy Lab, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.
Biofabrication ; 15(4)2023 09 01.
Article em En | MEDLINE | ID: mdl-37552982
Age-related musculoskeletal disorders, including osteoporosis, are frequent and associated with long lasting morbidity, in turn significantly impacting on healthcare system sustainability. There is therefore a compelling need to develop reliable preclinical models of disease and drug screening to validate novel drugs possibly on a personalized basis, without the need ofin vivoassay. In the context of bone tissue, although the osteocyte (Oc) network is a well-recognized therapeutic target, currentin vitropreclinical models are unable to mimic its physiologically relevant and highly complex structure. To this purpose, several features are needed, including an osteomimetic extracellular matrix, dynamic perfusion, and mechanical cues (e.g. shear stress) combined with a three-dimensional (3D) culture of Oc. Here we describe, for the first time, a high throughput microfluidic platform based on 96-miniaturized chips for large-scale preclinical evaluation to predict drug efficacy. We bioengineered a commercial microfluidic device that allows real-time visualization and equipped with multi-chips by the development and injection of a highly stiff bone-like 3D matrix, made of a blend of collagen-enriched natural hydrogels loaded with hydroxyapatite nanocrystals. The microchannel, filled with the ostemimetic matrix and Oc, is subjected to passive perfusion and shear stress. We used scanning electron microscopy for preliminary material characterization. Confocal microscopy and fluorescent microbeads were used after material injection into the microchannels to detect volume changes and the distribution of cell-sized objects within the hydrogel. The formation of a 3D dendritic network of Oc was monitored by measuring cell viability, evaluating phenotyping markers (connexin43, integrin alpha V/CD51, sclerostin), quantification of dendrites, and responsiveness to an anabolic drug. The platform is expected to accelerate the development of new drug aimed at modulating the survival and function of osteocytes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteócitos / Osso e Ossos Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteócitos / Osso e Ossos Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article