Investigating the Allometric Relationship for Total Mercury in Male and Female Signal Crayfish (Pacifastacus leniusculus) from Three Regions in the Columbia River Basin, USA.

The objective of this study was to evaluate the relationship between clawless body mass (CBM) and total mercury concentration (THg) in male and female crayfish captured from three different sites in the Columbia River Basin: the Spokane River, Boise River and Hangman Creek. It was found that the allometric relationships for THg in male and female crayfish did not differ from one another at any of the three locations (Spokane River: ANCOVA, F1,49 = 0.001, p = 0.63; Hangman Creek: ANCOVA, F1,73 = 0.007, p = 0.93; Boise River: ANCOVA, F1,38 = 0.02, p = 0.88. Furthermore, the slopes of the CBM and THg regression lines were not significantly different from one site to the next (ANCOVA, F2,166 = 1.5 p = 0.24), despite considerable differences in mean mass-adjusted THg across locations (Spokane River: 26.8 ± 2.6; Hangman Creek: 75.2 ± 2.1; Boise River: 99.0 ± 2.9). For signal crayfish (Pacifastacus leniusculus) from the Columbia River Basin, size and sex standardization can be accomplished with a single linear relationship.