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Fumigaclavine C ameliorates liver steatosis by attenuating hepatic de novo lipogenesis via modulation of the RhoA/ROCK signaling pathway.
Yu, Wanguo; Gao, Yaxin; Zhao, Zaoya; Long, Xiufeng; Yi, Yi; Ai, Shuo.
Afiliação
  • Yu W; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, 268 Donghuan Road, Liuzhou, 545006, People's Republic of China. yuwanguo@hotmail.com.
  • Gao Y; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, 268 Donghuan Road, Liuzhou, 545006, People's Republic of China. yuwanguo@hotmail.com.
  • Zhao Z; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, 268 Donghuan Road, Liuzhou, 545006, People's Republic of China.
  • Long X; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, 268 Donghuan Road, Liuzhou, 545006, People's Republic of China.
  • Yi Y; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, 268 Donghuan Road, Liuzhou, 545006, People's Republic of China.
  • Ai S; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, 268 Donghuan Road, Liuzhou, 545006, People's Republic of China.
BMC Complement Med Ther ; 23(1): 288, 2023 Aug 16.
Article em En | MEDLINE | ID: mdl-37587459
ABSTRACT

BACKGROUND:

Non-alcoholic fatty liver disease (NAFLD) has been well defined as a common chronic liver metabolism disorder. Statins as a first-line therapeutic treatment had some side effects. Here, we found that Fumigaclavine C (FC) was collected from endophytic Aspergillus terreus via the root of Rhizophora stylosa (Rhizophoraceae), had potential anti-adipogenic and hepatoprotective effects both in vitro and in vivo without obvious adverse side effects. However, the mechanisms of the prevention and management of FC for hepatic steatosis are incompletely delineated.

METHODS:

The pharmacodynamic effects of FC were measured in high-fat diet (HFD)-induced obese mice. Liver index and blood biochemical were examined. Histopathological examination in the liver was performed by hematoxylin & eosin or oil red O. The levels of serum TG, TC, LDL-c, HDL-c, FFA, T-bili, ALT, AST, creatinine, and creatine kinase were estimated via diagnostic assay kits. The levels of hepatic lipid metabolism-related genes were detected via qRT-PCR. The expression levels of hepatic de novo lipogenesis were quantitated with Western blot analysis

RESULTS:

FC-treatment markedly reduced hepatic lipid accumulation in HFD-induced obese mice. FC significantly attenuated the hepatic lipid metabolism and ameliorated liver injury without obvious adverse side effects. Moreover, FC also could dose-dependently modulate the expressions of lipid metabolism-related transcription genes. Mechanically, FC notably suppressed sterol response element binding protein-1c mediated de novo lipogenesis via interfering with the RhoA/ROCK signaling pathway by decreasing the levels of geranylgeranyl diphosphate and farnesyl diphosphate.

CONCLUSIONS:

These findings suggested that FC could improve hepatic steatosis through inhibiting de novo lipogenesis via modulating the RhoA/ROCK signaling pathway.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Alcaloides Indólicos / Alcaloides de Claviceps / Hepatopatia Gordurosa não Alcoólica Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Alcaloides Indólicos / Alcaloides de Claviceps / Hepatopatia Gordurosa não Alcoólica Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article