Chemical synthesis of on demand-activated SUMO-based probe by a photocaged glycine-assisted strategy.
Bioorg Med Chem Lett
; 94: 129460, 2023 10 01.
Article
em En
| MEDLINE
| ID: mdl-37640164
ABSTRACT
The transiently-activated SUMO probes are conducive to understand the dynamic control of SENPs activity. Here, we developed a photocaged glycine-assisted strategy for the construction of on demand-activated SUMO-ABPs. The light-sensitive groups installed at G92 and G64 backbone of SUMO-2 can temporarily block probes activity and hamper aspartimide formation, respectively, which enabled the efficient synthesis of inert SUMO-2 propargylamide (PA). The probe could be activated to capture SENPs upon photo-irradiation not only in vitro but also in intact cells, providing opportunities to further perform intracellular time-resolved proteome-wide profiling of SUMO-related enzymes.
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Base de dados:
MEDLINE
Assunto principal:
Sondas Moleculares
/
Proteína SUMO-1
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article