Ultrasensitive digital immunoassays for SOD1 conformation in amyotrophic lateral sclerosis.

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease associated with protein misfolding, including Cu/Zn SOD1. In this study, we set up a method for detecting normal and pathological misfolded SOD1 in human serum and cerebrospinal fluid. SOD1 was increased in ALS and misfolded SOD1 had low and variable expression in both control and ALS. These assays holds promise for improving our understanding of ALS and its diagnosis.