Symmetric inheritance of parental histones governs epigenome maintenance and embryonic stem cell identity.
Nat Genet
; 55(9): 1567-1578, 2023 09.
Article
em En
| MEDLINE
| ID: mdl-37666988
ABSTRACT
Modified parental histones are segregated symmetrically to daughter DNA strands during replication and can be inherited through mitosis. How this may sustain the epigenome and cell identity remains unknown. Here we show that transmission of histone-based information during DNA replication maintains epigenome fidelity and embryonic stem cell plasticity. Asymmetric segregation of parental histones H3-H4 in MCM2-2A mutants compromised mitotic inheritance of histone modifications and globally altered the epigenome. This included widespread spurious deposition of repressive modifications, suggesting elevated epigenetic noise. Moreover, H3K9me3 loss at repeats caused derepression and H3K27me3 redistribution across bivalent promoters correlated with misexpression of developmental genes. MCM2-2A mutation challenged dynamic transitions in cellular states across the cell cycle, enhancing naïve pluripotency and reducing lineage priming in G1. Furthermore, developmental competence was diminished, correlating with impaired exit from pluripotency. Collectively, this argues that epigenetic inheritance of histone modifications maintains a correctly balanced and dynamic chromatin landscape able to support mammalian cell differentiation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Histonas
/
Epigenoma
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article