A single-nucleotide resolution capillary gel electrophoresis workflow for poly(A) tail characterization in the development of mRNA therapeutics and vaccines.

Di Grandi, Deanna; Dayeh, Daniel M; Kaur, Keerat; Chen, Yizhuo; Henderson, Steven; Moon, Youmi; Bhowmick, Arijit; Ihnat, Peter M; Fu, Yue; Muthusamy, Kathir; Palackal, Nisha; Pyles, Erica A

Di Grandi, Deanna; Dayeh, Daniel M; Kaur, Keerat; Chen, Yizhuo; Henderson, Steven; Moon, Youmi; Bhowmick, Arijit; Ihnat, Peter M; Fu, Yue; Muthusamy, Kathir; Palackal, Nisha; Pyles, Erica A.

Afiliação

Di Grandi D; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA.
Dayeh DM; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA.
Kaur K; Regeneron Genetic Medicines, Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA.
Chen Y; Regeneron Genetic Medicines, Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA.
Henderson S; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA.
Moon Y; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA.
Bhowmick A; Regeneron Genetic Medicines, Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA.
Ihnat PM; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA.
Fu Y; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA. Electronic address: yue.fu@regeneron.com.
Muthusamy K; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA. Electronic address: kathir.muthusamy@regeneron.com.
Palackal N; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA.
Pyles EA; Protein Biochemistry, Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA.

J Pharm Biomed Anal ; 236: 115692, 2023 Nov 30.

Article em En | MEDLINE | ID: mdl-37696189

Assuntos

Nucleotídeos; Vacinas; RNA Mensageiro/genética; Fluxo de Trabalho; Eletroforese Capilar/métodos

Palavras-chave

Anion exchange liquid chromatography (AEX-LC); Capillary gel electrophoresis (CGE); In vitro transcribed messenger RNA (IVT mRNA); Ion-pair reversed-phase liquid chromatography (IPRP-LC); Poly(A) tail; Single-nucleotide resolution

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vacinas / Nucleotídeos Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vacinas / Nucleotídeos Idioma: En Ano de publicação: 2023 Tipo de documento: Article