Structural and antigenic characterization of the avian adeno-associated virus capsid.
J Virol
; 97(10): e0078023, 2023 10 31.
Article
em En
| MEDLINE
| ID: mdl-37702486
ABSTRACT
IMPORTANCE AAVs are extensively studied as promising therapeutic gene delivery vectors. In order to circumvent pre-existing antibodies targeting primate-based AAV capsids, the AAAV capsid was evaluated as an alternative to primate-based therapeutic vectors. Despite the high sequence diversity, the AAAV capsid was found to bind to a common glycan receptor, terminal galactose, which is also utilized by other AAVs already being utilized in gene therapy trials. However, contrary to the initial hypothesis, AAAV was recognized by approximately 30% of human sera tested. Structural and sequence comparisons point to conserved epitopes in the fivefold region of the capsid as the reason determinant for the observed cross-reactivity.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Capsídeo
/
Parvovirinae
/
Antígenos Virais
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article