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The Characteristics of Macrophage Heterogeneity in Atherosclerotic Aortas.
Song, Yu-Jie; Ma, Yu; Meng, Ting; Zhuang, Tao; Ruan, Cheng-Chao; Li, Yan; Zhang, Guan-Nan.
Afiliação
  • Song YJ; Department of Cardiovascular Medicine, State Key Laboratory of Medical Genomics, Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • Ma Y; Department of Cardiovascular Medicine, State Key Laboratory of Medical Genomics, Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • Meng T; Department of Physiology and Pathophysiology, Shanghai Key Laboratory of Bioactive Small Molecules, School of Basic Medical Sciences, and Jinshan Hospital, Fudan University, Shanghai, China.
  • Zhuang T; Department of Physiology and Pathophysiology, Shanghai Key Laboratory of Bioactive Small Molecules, School of Basic Medical Sciences, and Jinshan Hospital, Fudan University, Shanghai, China.
  • Ruan CC; Department of Physiology and Pathophysiology, Shanghai Key Laboratory of Bioactive Small Molecules, School of Basic Medical Sciences, and Jinshan Hospital, Fudan University, Shanghai, China.
  • Li Y; Department of Cardiology, RuiJin Hospital/LuWan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, China. liyan1987123@126.com.
  • Zhang GN; Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu, China. gnzhang@njmu.edu.cn.
J Cardiovasc Transl Res ; 17(1): 153-166, 2024 Feb.
Article em En | MEDLINE | ID: mdl-37713049
ABSTRACT
Macrophage is the main effector cell during atherosclerosis. We applied single-cell RNA sequencing (scRNA) data to investigate the role of macrophage subsets in atherosclerosis. Monocyte and macrophage clusters were divided into 6 subclusters. Each subcluster's markers were calculated and validated by immunofluorescence. Elevated macrophage subclusters in the WD group were subject to enrichment pathway analysis and exhibited different phenotypes. Pseudotime analysis shows the subclusters originate from monocytes. We cultured bone marrow-derived macrophages with CSF-1 and ox-LDL to simulate an atherosclerotic-like environment and detected the transformation of subclusters. Macrophage-Vegfa and Macrophage-C1qb increased in the WD group. Macrophage-Vegfa acquires the characteristics of phagocytosis and immune response, while Macrophage-C1qb is not involved in lipid metabolism. The two subclusters are both enriched in cell movement and migration pathways. Experimental verification proved Monocyte-Ly6C evolved into Macrophage-Vegfa and Macrophage-C1qb during atherosclerosis progression.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças da Aorta / Aterosclerose / Placa Aterosclerótica Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças da Aorta / Aterosclerose / Placa Aterosclerótica Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article