Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides.
Nat Commun
; 14(1): 6072, 2023 09 28.
Article
em En
| MEDLINE
| ID: mdl-37770474
ABSTRACT
Leaf rust, caused by Puccinia triticina Eriksson (Pt), is one of the most severe foliar diseases of wheat. Breeding for leaf rust resistance is a practical and sustainable method to control this devastating disease. Here, we report the identification of Lr47, a broadly effective leaf rust resistance gene introgressed into wheat from Aegilops speltoides. Lr47 encodes a coiled-coil nucleotide-binding leucine-rich repeat protein that is both necessary and sufficient to confer Pt resistance, as demonstrated by loss-of-function mutations and transgenic complementation. Lr47 introgression lines with no or reduced linkage drag are generated using the Pairing homoeologous1 mutation, and a diagnostic molecular marker for Lr47 is developed. The coiled-coil domain of the Lr47 protein is unable to induce cell death, nor does it have self-protein interaction. The cloning of Lr47 expands the number of leaf rust resistance genes that can be incorporated into multigene transgenic cassettes to control this devastating disease.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Basidiomycota
/
Aegilops
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article