Polymerase incorporation of fluorescein or rhodamine modified 2'-deoxyuridine-5'-triphosphates into double-stranded DNA for direct electrochemical detection.

Suprun, Elena V; Khmeleva, Svetlana A; Bibik, Konstantin V; Ptitsyn, Konstantin G; Kurbatov, Leonid K; Radko, Sergey P

Suprun, Elena V; Khmeleva, Svetlana A; Bibik, Konstantin V; Ptitsyn, Konstantin G; Kurbatov, Leonid K; Radko, Sergey P.

Afiliação

Suprun EV; Chemistry Faculty of M.V. Lomonosov Moscow State University, Lenin Hills, 1/3, Moscow 119991, Russia; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow 119121, Russia. Electronic address: lenasuprun@mail.ru.
Khmeleva SA; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow 119121, Russia.
Bibik KV; Chemistry Faculty of M.V. Lomonosov Moscow State University, Lenin Hills, 1/3, Moscow 119991, Russia; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow 119121, Russia.
Ptitsyn KG; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow 119121, Russia.
Kurbatov LK; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow 119121, Russia.
Radko SP; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, Moscow 119121, Russia.

J Pharm Biomed Anal ; 236: 115737, 2023 Nov 30.

Article em En | MEDLINE | ID: mdl-37774487

Assuntos

DNA; Desoxiuridina; Rodaminas; Fluoresceína; DNA/análise; Reação em Cadeia da Polimerase

Palavras-chave

Fluorescein; Modified nucleotide; Polymerase chain reaction; Recombinase polymerase amplification; Rhodamine; Voltammetric detection

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Desoxiuridina Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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