Pharmamedia and pectinase production by Bacillus subtilis Mz-12P.
Biotechnol Appl Biochem
; 71(1): 38-44, 2024 Feb.
Article
em En
| MEDLINE
| ID: mdl-37786232
ABSTRACT
Bacterial isolates collected from the environment were screened for pectinolytic activity, and a strain with the highest activity was selected and identified as Bacillus subtilis Mz-12. The presence of pectin hydrolase, lyase, and esterase activities was confirmed. Pectinase was purified and characterized. Enzyme production was optimized with respect to temperature, pH, and growth medium. Enzyme stability and activity were characterized under different temperatures and pH values. The results showed that this strain was capable of producing high yields of pectinase in commercial medium (Pharmamedia) 24.6 U/mL compared to other media. The purified pectinase of 22.3 kDa produced was constitutive in nature. The isolated enzyme from this strain displayed a wide range of temperature and pH stability, with the optimal activity observed at pH 9.0 and 50°C. These results indicate that the B. subtilis Mz-12 strain is a favorable candidate for industrial enzyme production. The use of Pharmamedia is reported for first time for pectinase production.
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MEDLINE
Assunto principal:
Poligalacturonase
/
Bacillus subtilis
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article