miR-624 accelerates the growth of liver cancer cells by inhibiting EMC3.
Noncoding RNA Res
; 8(4): 641-644, 2023 Dec.
Article
em En
| MEDLINE
| ID: mdl-37810370
miRNA is a noncoding RNA found in recent years and more than one third of human genes are the target of miRNAs. miR-624, located on human chromosome 14, is associated with tumorigenesis. However, the role of miR-624 in human hepatocarcinogenesis is still unclear. Herein, our results indicate that miR-624 accelerates the growth of liver cancer cells in vivo and in vitro. Moreover, the modification distribution of H3K9me1 on chromosomes is different between rLV group and rLV-miR-624 group. miR-624 affects epigenetic regulation of several genes in human liver cancer cells, such as RAB21, SMARCD3, MAPK6,PRRX1, ZFHX3, EMC3 (TMEM111). Furthermore, miR-624 affects transcriptome of some genes in liver cancer, including RAB21ï¼ UBE2Nï¼ PPP1CCï¼KPNA3ï¼ RAB7Aï¼CPEB2,KLF4ï¼ MARK2ï¼ JUNï¼ ARF6ï¼ TMEM39A. On the other hand, miR-624 affects proteome of several genes in liver cancer, such as, RBM5,PTK2, KDM2A,POLR2H, POLR2G,CDK6,KIF15,CUL2,FKBP2,ErbB-3,JUN, PKM2, CyclinE,PLK1, mTOR, PPARγ, Rab7A,ARAF, UPF3B ï¼PTEN, SUZ12, GADD45, H3.3, CUL5, ARF6,EMC3,ATG4B,ATG14,CALR. Interestingly, miR-624 affects the RAB7A interaction network in liver cancer cells, involving in CLTCï¼ITGB1ï¼HNRNPUï¼ DARS1ï¼ RPS16ï¼ CTPS1ï¼H3-3Bï¼JUN,MYH10ï¼ CUL5ï¼ CPSF7. Strikingly, excessive MEC3 abrogates the carcinogenic functions of miR-624. Importantly, our findings indicate that miR-624 affects some signaling pathway in liver cancer, including Wnt signaling pathwayï¼Hippo signaling pathwayï¼mTOR signaling pathway, Ras signaling pathwayï¼MAPK signaling pathwayï¼PI3K-Akt signaling pathway, erbB signaling pathway. These results provide a basis for the treatment of human liver cancer.
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MEDLINE
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En
Ano de publicação:
2023
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Article