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Intraoperative Integrated Diagnostic System for Malignant Central Nervous System Tumors.
Hayashi, Takahiro; Tateishi, Kensuke; Matsuyama, Shinichiro; Iwashita, Hiromichi; Miyake, Yohei; Oshima, Akito; Honma, Hirokuni; Sasame, Jo; Takabayashi, Katsuhiro; Sugino, Kyoka; Hirata, Emi; Udaka, Naoko; Matsushita, Yuko; Kato, Ikuma; Hayashi, Hiroaki; Nakamura, Taishi; Ikegaya, Naoki; Takayama, Yutaro; Sonoda, Masaki; Oka, Chihiro; Sato, Mitsuru; Isoda, Masataka; Kato, Miyui; Uchiyama, Kaho; Tanaka, Tamon; Muramatsu, Toshiki; Miyake, Shigeta; Suzuki, Ryosuke; Takadera, Mutsumi; Tatezuki, Junya; Ayabe, Junichi; Suenaga, Jun; Matsunaga, Shigeo; Miyahara, Kosuke; Manaka, Hiroshi; Murata, Hidetoshi; Yokoyama, Takaakira; Tanaka, Yoshihide; Shuto, Takashi; Ichimura, Koichi; Kato, Shingo; Yamanaka, Shoji; Cahill, Daniel P; Fujii, Satoshi; Shankar, Ganesh M; Yamamoto, Tetsuya.
Afiliação
  • Hayashi T; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Tateishi K; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Matsuyama S; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Iwashita H; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Miyake Y; Laboratory of Biopharmaceutical and Regenerative Science, Graduate School of Medical Science, Yokohama City University, Yokohama, Japan.
  • Oshima A; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Honma H; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Sasame J; Department of Pathology, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Takabayashi K; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Sugino K; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Hirata E; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Udaka N; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Matsushita Y; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Kato I; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Hayashi H; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Nakamura T; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Ikegaya N; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Takayama Y; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Sonoda M; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Oka C; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Sato M; Laboratory of Biopharmaceutical and Regenerative Science, Graduate School of Medical Science, Yokohama City University, Yokohama, Japan.
  • Isoda M; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Kato M; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Uchiyama K; Department of Diagnostic Pathology, Yokohama City University Hospital, Yokohama, Japan.
  • Tanaka T; Department of Brain Disease Translational Research, Juntendo University Faculty of Medicine, Tokyo, Japan.
  • Muramatsu T; Department of Molecular Pathology, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Miyake S; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Suzuki R; Department of Pediatrics, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Takadera M; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Tatezuki J; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Ayabe J; Department of Neurosurgery, Yokohama City University Medical Center, Yokohama, Japan.
  • Suenaga J; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Matsunaga S; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Miyahara K; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Manaka H; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Murata H; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Yokoyama T; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Tanaka Y; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Shuto T; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Ichimura K; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
  • Kato S; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Yamanaka S; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Cahill DP; Laboratory of Biopharmaceutical and Regenerative Science, Graduate School of Medical Science, Yokohama City University, Yokohama, Japan.
  • Fujii S; Neurosurgical-Oncology Laboratory, Yokohama City University, Yokohama, Japan.
  • Shankar GM; Laboratory of Biopharmaceutical and Regenerative Science, Graduate School of Medical Science, Yokohama City University, Yokohama, Japan.
  • Yamamoto T; Department of Neurosurgery, Yokohama City University, Graduate School of Medicine, Yokohama, Japan.
Clin Cancer Res ; 30(1): 116-126, 2024 01 05.
Article em En | MEDLINE | ID: mdl-37851071
ABSTRACT

PURPOSE:

The 2021 World Health Organization (WHO) classification of central nervous system (CNS) tumors uses an integrated approach involving histopathology and molecular profiling. Because majority of adult malignant brain tumors are gliomas and primary CNS lymphomas (PCNSL), rapid differentiation of these diseases is required for therapeutic decisions. In addition, diffuse gliomas require molecular information on single-nucleotide variants (SNV), such as IDH1/2. Here, we report an intraoperative integrated diagnostic (i-ID) system to classify CNS malignant tumors, which updates legacy frozen-section (FS) diagnosis through incorporation of a qPCR-based genotyping assay. EXPERIMENTAL

DESIGN:

FS evaluation, including GFAP and CD20 rapid IHC, was performed on adult malignant CNS tumors. PCNSL was diagnosed through positive CD20 and negative GFAP immunostaining. For suspected glioma, genotyping for IDH1/2, TERT SNV, and CDKN2A copy-number alteration was routinely performed, whereas H3F3A and BRAF SNV were assessed for selected cases. i-ID was determined on the basis of the 2021 WHO classification and compared with the permanent integrated diagnosis (p-ID) to assess its reliability.

RESULTS:

After retrospectively analyzing 153 cases, 101 cases were prospectively examined using the i-ID system. Assessment of IDH1/2, TERT, H3F3AK27M, BRAFV600E, and CDKN2A alterations with i-ID and permanent genomic analysis was concordant in 100%, 100%, 100%, 100%, and 96.4%, respectively. Combination with FS and intraoperative genotyping assay improved diagnostic accuracy in gliomas. Overall, i-ID matched with p-ID in 80/82 (97.6%) patients with glioma and 18/19 (94.7%) with PCNSL.

CONCLUSIONS:

The i-ID system provides reliable integrated diagnosis of adult malignant CNS tumors.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Encefálicas / Neoplasias do Sistema Nervoso Central / Glioma Limite: Adult / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Encefálicas / Neoplasias do Sistema Nervoso Central / Glioma Limite: Adult / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article