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Ethanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue.
Borges, Morgana A; Curcio, Bruna R; Gastal, Gustavo D A; Gheno, Luiza; Junior, Antonio S Varela; Corcini, Carine D; Nogueira, Carlos E W; Aguiar, Francisco L N; Gastal, Eduardo L.
Afiliação
  • Borges MA; Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Curcio BR; Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Gastal GDA; Instituto Nacional de Investigación Agropecuaria, Estación Experimental INIA La Estanzuela, Colonia, Uruguay.
  • Gheno L; Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Junior ASV; Department of Comparative Animal Reproduction, Institute of Biological Sciences, Federal University of Rio Grande, Rio Grande, RS, Brazil.
  • Corcini CD; Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Nogueira CEW; Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Aguiar FLN; Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraiba, Sousa, PB, Brazil.
  • Gastal EL; Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, USA. Electronic address: egastal@siu.edu.
Reprod Biol ; 23(4): 100814, 2023 Dec.
Article em En | MEDLINE | ID: mdl-37890396
The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ovário / Folículo Ovariano Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ovário / Folículo Ovariano Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article