Viral vector-mediated transgene delivery with novel recombinase systems for targeting neuronal populations defined by multiple features.
Neuron
; 112(1): 56-72.e4, 2024 Jan 03.
Article
em En
| MEDLINE
| ID: mdl-37909037
ABSTRACT
A comprehensive understanding of neuronal diversity and connectivity is essential for understanding the anatomical and cellular mechanisms that underlie functional contributions. With the advent of single-cell analysis, growing information regarding molecular profiles leads to the identification of more heterogeneous cell types. Therefore, the need for additional orthogonal recombinase systems is increasingly apparent, as heterogeneous tissues can be further partitioned into increasing numbers of specific cell types defined by multiple features. Critically, new recombinase systems should work together with pre-existing systems without cross-reactivity in vivo. Here, we introduce novel site-specific recombinase systems based on ΦC31 bacteriophage recombinase for labeling multiple cell types simultaneously and a novel viral strategy for versatile and robust intersectional expression of any transgene. Together, our system will help researchers specifically target different cell types with multiple features in the same animal.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Integrases
/
Recombinases
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article