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Amnion responses to intrauterine inflammation and effects of inhibition of TNF signaling in preterm Rhesus macaque.
Presicce, Pietro; Cappelletti, Monica; Morselli, Marco; Ma, Feiyang; Senthamaraikannan, Paranthaman; Protti, Giulia; Nadel, Brian B; Aryan, Laila; Eghbali, Mansoureh; Salwinski, Lukasz; Pithia, Neema; De Franco, Emily; Miller, Lisa A; Pellegrini, Matteo; Jobe, Alan H; Chougnet, Claire A; Kallapur, Suhas G.
Afiliação
  • Presicce P; Divisions of Neonatology and Developmental Biology, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA, USA.
  • Cappelletti M; Divisions of Neonatology and Developmental Biology, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA, USA.
  • Morselli M; Department of Molecular, Cell and Developmental Biology Medicine at the University of California Los Angeles, Los Angeles, CA, USA.
  • Ma F; Institute for Quantitative and Computational Biosciences - Collaboratory at the University of California Los Angeles, Los Angeles, CA, USA.
  • Senthamaraikannan P; Department of Molecular, Cell and Developmental Biology Medicine at the University of California Los Angeles, Los Angeles, CA, USA.
  • Protti G; Institute for Quantitative and Computational Biosciences - Collaboratory at the University of California Los Angeles, Los Angeles, CA, USA.
  • Nadel BB; Division of Neonatology/Pulmonary Biology, Cincinnati Children's Hospital Research Foundation, The University of Cincinnati College of Medicine, Cincinnati, OH, USA.
  • Aryan L; Division of Immunobiology, Cincinnati Children's Hospital Research Foundation, The University of Cincinnati College of Medicine, Cincinnati, OH, USA.
  • Eghbali M; Institute for Quantitative and Computational Biosciences - Collaboratory at the University of California Los Angeles, Los Angeles, CA, USA.
  • Salwinski L; Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy.
  • Pithia N; Department of Molecular Cellular and Developmental Biology, and Institute for Genomics and Proteomics, University of California Los Angeles, Los Angeles, CA, USA.
  • De Franco E; California National Primate Research Center, University of California Davis, Davis, CA, USA.
  • Miller LA; Division of Molecular Medicine, Department of Anesthesiology, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA, USA.
  • Pellegrini M; Division of Molecular Medicine, Department of Anesthesiology, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA, USA.
  • Jobe AH; UCLA-DOE Institute of Genomics and Proteomics, University of California Los Angeles, Los Angeles, CA, USA.
  • Chougnet CA; Divisions of Neonatology and Developmental Biology, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA, USA.
  • Kallapur SG; Department of Obstetrics/Gynecology, Maternal-Fetal Medicine, University of Cincinnati, Cincinnati, OH, USA.
iScience ; 26(11): 108118, 2023 Nov 17.
Article em En | MEDLINE | ID: mdl-37953944
ABSTRACT
Intrauterine infection/inflammation (IUI) is a frequent complication of pregnancy leading to preterm labor and fetal inflammation. How inflammation is modulated at the maternal-fetal interface is unresolved. We compared transcriptomics of amnion (a fetal tissue in contact with amniotic fluid) in a preterm Rhesus macaque model of IUI induced by lipopolysaccharide with human cohorts of chorioamnionitis. Bulk RNA sequencing (RNA-seq) amnion transcriptomic profiles were remarkably similar in both Rhesus and human subjects and revealed that induction of key labor-mediating genes such as IL1 and IL6 was dependent on nuclear factor κB (NF-κB) signaling and reversed by the anti-tumor necrosis factor (TNF) antibody Adalimumab. Inhibition of collagen biosynthesis by IUI was partially restored by Adalimumab. Interestingly, single-cell transcriptomics, flow cytometry, and immunohistology demonstrated that a subset of amnion mesenchymal cells (AMCs) increase CD14 and other myeloid cell markers during IUI both in the human and Rhesus macaque. Our data suggest that CD14+ AMCs represent activated AMCs at the maternal-fetal interface.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article