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First molecular characterization of Dirofilaria Immitis in Cuba.
Roblejo-Arias, Lisset; Díaz-Corona, Cristian; Piloto-Sardiñas, Elianne; Díaz-Sánchez, Adrian A; Zajac, Zbigniew; Kulisz, Joanna; Wozniak, Aneta; Moutailler, Sara; Obregon, Dasiel; Foucault-Simonin, Angélique; Corona-González, Belkis; Cabezas-Cruz, Alejandro.
Afiliação
  • Roblejo-Arias L; Direction of Animal Health, National Center for Animal and Plant Health, Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas, Mayabeque, 32700, Cuba.
  • Díaz-Corona C; Direction of Animal Health, National Center for Animal and Plant Health, Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas, Mayabeque, 32700, Cuba.
  • Piloto-Sardiñas E; Direction of Animal Health, National Center for Animal and Plant Health, Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas, Mayabeque, 32700, Cuba.
  • Díaz-Sánchez AA; Laboratoire de Santé Animale, ANSES, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Maisons-Alfort, France.
  • Zajac Z; University of Saskatchewan, Saskatchewan, Canada.
  • Kulisz J; Department of Biology and Parasitology, Medical University of Lublin, Radziwillowska 11st, Lublin, 20-080, Poland.
  • Wozniak A; Department of Biology and Parasitology, Medical University of Lublin, Radziwillowska 11st, Lublin, 20-080, Poland.
  • Moutailler S; Department of Biology and Parasitology, Medical University of Lublin, Radziwillowska 11st, Lublin, 20-080, Poland.
  • Obregon D; Laboratoire de Santé Animale, ANSES, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Maisons-Alfort, France.
  • Foucault-Simonin A; School of Environmental Sciences, University of Guelph, Guelph, ON, Canada.
  • Corona-González B; Laboratoire de Santé Animale, ANSES, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Maisons-Alfort, France.
  • Cabezas-Cruz A; Direction of Animal Health, National Center for Animal and Plant Health, Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas, Mayabeque, 32700, Cuba. bcorogonz@gmail.com.
BMC Vet Res ; 19(1): 239, 2023 Nov 17.
Article em En | MEDLINE | ID: mdl-37978522
BACKGROUND: Dirofilarioses are widespread diseases caused by mosquito-borne nematodes of the family Onchocercidae, genus Dirofilaria. The major etiologic agent of canine dirofilariosis in the American continent is the zoonotic parasite Dirofilaria immitis. Existing reports of filarioid nematodes in Cuba are based solely on morphological and immunological analysis which do not allow unambiguous identification and/or direct detection of causal agents. RESULTS: Here we present the molecular characterization of filarioid nematodes found in a dog in Cuba. Based on the molecular and phylogenetic analysis of the 5.8S-ITS2-28S region and cox1 gene fragments, the worms were unambiguously classified as D. immitis. Sequence analysis showed high identity of the gene fragments in this study with others previously obtained from D. immitis found in dogs, wolfs and jackals but also from mosquito vectors of D. immitis. CONCLUSIONS: Further studies are guarantee to better understand the epidemiological impact of canine dirofilariosis in Cuba as well as the competence of different species of culicid mosquitoes as vectors of Dirofilaria in the country.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dirofilaria immitis / Dirofilariose / Doenças do Cão / Dirofilaria repens / Culicidae Limite: Animals País como assunto: Caribe / Cuba Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dirofilaria immitis / Dirofilariose / Doenças do Cão / Dirofilaria repens / Culicidae Limite: Animals País como assunto: Caribe / Cuba Idioma: En Ano de publicação: 2023 Tipo de documento: Article