scMAR-Seq: a novel workflow for targeted single-cell genomics of microorganisms using radioactive labeling.
mSystems
; 8(6): e0099823, 2023 Dec 21.
Article
em En
| MEDLINE
| ID: mdl-37982643
ABSTRACT
IMPORTANCE A central question in microbial ecology is which member of a community performs a particular metabolism. Several sophisticated isotope labeling techniques are available for analyzing the metabolic function of populations and individual cells in a community. However, these methods are generally either insufficiently sensitive or throughput-limited and thus have limited applicability for the study of complex environmental samples. Here, we present a novel approach that combines highly sensitive radioisotope tracking, microfluidics, high-throughput sorting, and single-cell genomics to simultaneously detect and identify individual microbial cells based solely on their in situ metabolic activity, without prior information on community structure.
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1
Base de dados:
MEDLINE
Assunto principal:
Genômica
/
Microfluídica
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article