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Molecular Detection of Hemoplasma in animals in Tamil Nadu, India and Hemoplasma genome analysis.
Murugesan, Ananda Chitra; Kumaragurubaran, Karthik; Gunasekaran, Kavin; Murugasamy, Sabari Arul; Arunachalam, Shanmugasundaram; Annamalai, Raman; Ragothaman, Venkataramanan; Ramaswamy, Sridhar.
Afiliação
  • Murugesan AC; Central University Laboratory, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, 600051, Tamil Nadu, India. anandachitra.m@tanuvas.ac.in.
  • Kumaragurubaran K; Department of Veterinary Microbiology, Veterinary College and Research Institute, Udumalpet, 642126, Tamil Nadu, India.
  • Gunasekaran K; Veterinary Dispensary, Poompuhar, 609105, Tamil Nadu, India.
  • Murugasamy SA; Veterinary Dispensary, Poompuhar, 609105, Tamil Nadu, India.
  • Arunachalam S; Livestock Farm Complex, TANUVAS, Madhavaram Milk Colony, Chennai, 600051, Tamil Nadu, India.
  • Annamalai R; Animal Disease Intelligent Unit, Thiruvannamalai, 606601, Tamil Nadu, India.
  • Ragothaman V; Livestock Farm Complex, TANUVAS, Madhavaram Milk Colony, Chennai, 600051, Tamil Nadu, India.
  • Ramaswamy S; Central University Laboratory, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, 600051, Tamil Nadu, India.
Vet Res Commun ; 48(2): 955-968, 2024 Apr.
Article em En | MEDLINE | ID: mdl-38032521
ABSTRACT
Hemoplasma are small pleomorphic wall-less Gram-positive bacteria that infect erythrocytes of various mammalian hosts. They generally cause asymptomatic or chronic anaemia but occasionally causes overt life-threatening hemolytic anaemia. In the present study, 316 cattle, 115 sheep, 61 goats and 6 buffalo blood samples were collected from various villages or organized farms located in nine districts of Tamil Nadu to detect the hemoplasma by PCR. Overall prevalence of 43.04%, 65.22%, and 44.26% hemoplasma DNA was observed in cattle, sheep and goats, respectively. In total, 21 hemoplasma positive samples were sequenced for 16S rRNA gene which revealed 8 Mycoplasma wenyonii, 11 'Candidatus Mycoplasma haemobos' and one Mycoplasma ovis infection. Sheep blood samples from Chennai district were infected with 'Ca. M. haemobos' whereas sheep sample from Thiruvannamalai district was infected with M. wenyonii. At least 50% genes in the hemoplasma genomes were paralogous genes whose functions were not known. Only 'Ca. M. haemolamae' genome contained one primitive CRISPR system without any cas genes. Antimicrobial resistance genes (ARG) could not be identified in any of the hemoplasma genomes but homologous ARG were identified in all the genomes. Adhesion related gene EF-Tu was detected in all 14 hemoplasma genomes but enolase gene was detected only in 'Ca. M. haemohominis' SWG34-3 genome. This is the first report on the prevalence of hemoplasma infection in cattle, sheep and goat in India.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças dos Ovinos / Doenças dos Bovinos / Doenças das Cabras / Anemia / Infecções por Mycoplasma Limite: Animals País como assunto: Asia Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças dos Ovinos / Doenças dos Bovinos / Doenças das Cabras / Anemia / Infecções por Mycoplasma Limite: Animals País como assunto: Asia Idioma: En Ano de publicação: 2024 Tipo de documento: Article