Effect of lncRNA LINC00324 on cervical cancer progression through down-regulation of miR-195-5p.

ABSTRACT

BACKGROUND: Long non-coding RNAs (lncRNAs) have been widely used in the exploration of diseases in recent years. This paper introduced the significance of lncRNA LINC00324 (LINC00324) on the progression of cervical cancer and explored the mechanism of action and potential prognosis of LINC00324. METHODS: The cervical cancer tissues and adjacent normal tissues of 120 people were collected as research samples. The expression level of LINC00324 was assessed by RT-qPCR, as was miR-195-5p. Knockdown of LINC00324 on the proliferation ability of cervical cancer cells was determined with the help of cell counting kit-8 (CCK-8), and the number of cell migration and invasion was detected by the Transwell method. Luciferase reporter gene assay was used to analyse the correlation of LINC00324 and miR-195-5p. Kaplan-Meier survival curves and multivariate Cox analysis explained the potential prognostic significance of LINC00324 in cervical cancer. RESULTS: Significantly increased expression of LINC00324 and down-regulated miR-195-5p were negatively correlated in cervical cancer. Knockdown of LINC00324 inhibited the progression of cervical cancer, which was related to its mechanism of targeting and downregulating miR-195-5p. In addition, low expression of LINC00324 may prolong the survival period of patients with cervical cancer. CONCLUSIONS: LINC00324 targets miR-195-5p to regulate the progression of cervical cancer and have potential as a prognostic molecular marker for cervical cancer.

This paper introduced the mechanism and prognostic potential of LINC00324 in cervical cancer. The study found that LINC00324 expression was significantly elevated, while miR-195-5p level was down-regulated in cervical cancer. LINC00324 sponging miR-195-5p regulated the proliferation, migration and invasion of cervical cancer cells, thereby affecting the progression of cervical cancer. LINC00324 may be a prognostic biomarker for cervical cancer, providing a new direction for the treatment of patients.