SAP30BP interacts with RBM17/SPF45 to promote splicing in a subset of human short introns.
Cell Rep
; 42(12): 113534, 2023 12 26.
Article
em En
| MEDLINE
| ID: mdl-38065098
ABSTRACT
Human pre-mRNA splicing requires the removal of introns with highly variable lengths, from tens to over a million nucleotides. Therefore, mechanisms of intron recognition and splicing are likely not universal. Recently, we reported that splicing in a subset of human short introns with truncated polypyrimidine tracts depends on RBM17 (SPF45), instead of the canonical splicing factor U2 auxiliary factor (U2AF) heterodimer. Here, we demonstrate that SAP30BP, a factor previously implicated in transcriptional control, is an essential splicing cofactor for RBM17. In vitro binding and nuclear magnetic resonance analyses demonstrate that a U2AF-homology motif (UHM) in RBM17 binds directly to a newly identified UHM-ligand motif in SAP30BP. We show that this RBM17-SAP30BP interaction is required to specifically recruit RBM17 to phosphorylated SF3B1 (SF3b155), a U2 small nuclear ribonucleoprotein (U2 snRNP) component in active spliceosomes. We propose a mechanism for splicing in a subset of short introns, in which SAP30BP guides RBM17 in the assembly of active spliceosomes.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Splicing de RNA
/
Spliceossomos
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article