Isolation, Purification, and Application of Protoplasts and Transient Expression Systems in Plants.
Int J Mol Sci
; 24(23)2023 Nov 29.
Article
em En
| MEDLINE
| ID: mdl-38069215
ABSTRACT
Protoplasts, derived from plant cells, exhibit remarkable totipotency and hold significant value across a wide spectrum of biological and biotechnological applications. These versatile applications encompass protein subcellular localization and interaction analysis, gene expression regulation, functional characterization, gene editing techniques, and single-cell sequencing. Protoplasts' usability stems from their inherent accessibility and their ability to efficiently incorporate exogenous genes. In this review, we provide a comprehensive overview, including details on isolation procedures and influencing factors, purification and viability assessment methodologies, and the utilization of the protoplast transient expression system. The aim is to provide a comprehensive overview of current applications and offer valuable insights into protoplast isolation and the establishment of transient expression systems in a diverse range of plant species, thereby serving as a valuable resource for the plant science community.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plantas
/
Protoplastos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article