Your browser doesn't support javascript.
loading
Multienzymatic disintegration of DNA-scaffolded magnetic nanoparticle assembly for malarial mitochondrial DNA detection.
Li, Tingting; Meng, Fanming; Fang, Yuan; Luo, Yifei; He, Yilong; Dong, Zhuxin; Tian, Bo.
Afiliação
  • Li T; School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
  • Meng F; School of Basic Medical Sciences, Central South University, Changsha, 410013, China; School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, 830017, China.
  • Fang Y; School of Basic Medical Sciences, Central South University, Changsha, 410013, China; College of Biology, Hunan University, Changsha, 410082, China.
  • Luo Y; School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
  • He Y; School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
  • Dong Z; School of Basic Medical Sciences, Central South University, Changsha, 410013, China; Furong Laboratory, Changsha, 410008, China.
  • Tian B; School of Basic Medical Sciences, Central South University, Changsha, 410013, China; Furong Laboratory, Changsha, 410008, China. Electronic address: tianbo@csu.edu.cn.
Biosens Bioelectron ; 246: 115910, 2024 Feb 15.
Article em En | MEDLINE | ID: mdl-38086308
ABSTRACT
Early diagnosis of malaria can prevent the spread of disease and save lives, which, however, remains challenging in remote and less developed regions. Here we report a portable and low-cost optomagnetic biosensor for rapid amplification and detection of malarial mitochondrial DNA. Bioresponsive magnetic nanoparticle assemblies are constructed by using nucleic acid scaffolds containing endonucleolytic DNAzymes and their substrates, which can be activated by the presence of target DNA and self-disintegrated to release magnetic nanoparticles for optomagnetic quantification. Specifically, target molecules can induce padlock probe ligation and subsequent one-pot homogeneous cascade reactions consisting of nicking-enhanced rolling circle amplification, DNAzyme-assisted nucleic acid recycling, and strand-displacement-driven disintegration of the magnetic assembly. With an optimized magnetic actuation process for reaction acceleration, a detection limit of 1 fM can be achieved by the proposed biosensor with a total assay time of ca. 90 min and a dynamic detection range spanning 3 orders of magnitude. The robustness of the system was validated by testing target molecules spiked in 5% serum samples. Clinical sample validation was conducted by testing malaria-positive clinical blood specimens, obtaining quantitative results concordant with qPCR measurements.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / DNA Catalítico / Nanopartículas de Magnetita / Malária Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / DNA Catalítico / Nanopartículas de Magnetita / Malária Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article