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Spatiotemporal transcriptome atlas reveals the regional specification of the developing human brain.
Li, Yanxin; Li, Zhongqiu; Wang, Changliang; Yang, Min; He, Ziqing; Wang, Feiyang; Zhang, Yuehong; Li, Rong; Gong, Yunxia; Wang, Binhong; Fan, Baoguang; Wang, Chunyue; Chen, Lei; Li, Hong; Shi, Peifu; Wang, Nana; Wei, Zhifeng; Wang, Yan-Ling; Jin, Lei; Du, Peng; Dong, Ji; Jiao, Jianwei.
Afiliação
  • Li Y; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Medical School, University of Chinese Academy of Sciences, Beijing 100049, China; Beijing Institute for Stem Cell and Regenerative Medicine, Institute for Stem Cell a
  • Li Z; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Medical School, University of Chinese Academy of Sciences, Beijing 100049, China; Beijing Institute for Stem Cell and Regenerative Medicine, Institute for Stem Cell a
  • Wang C; GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macau Joint Laboratory for Cell Fate Regulation and Diseases, Guangzhou National Laboratory, Guangzhou Medical University, Guangzhou 510799, China.
  • Yang M; MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University, Beijing 100871, China.
  • He Z; GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macau Joint Laboratory for Cell Fate Regulation and Diseases, Guangzhou National Laboratory, Guangzhou Medical University, Guangzhou 510799, China; Faculty of Health Sciences University of Macau, Macau 999078, China.
  • Wang F; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Medical School, University of Chinese Academy of Sciences, Beijing 100049, China; Beijing Institute for Stem Cell and Regenerative Medicine, Institute for Stem Cell a
  • Zhang Y; Tongzhou Maternal and Child Health Hospital of Beijing, Beijing 101100, China.
  • Li R; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Department of Obstetrics and Gynecology, Ministry of Education, Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China; National C
  • Gong Y; Tongzhou Maternal and Child Health Hospital of Beijing, Beijing 101100, China.
  • Wang B; Tongzhou Maternal and Child Health Hospital of Beijing, Beijing 101100, China.
  • Fan B; Tongzhou Maternal and Child Health Hospital of Beijing, Beijing 101100, China.
  • Wang C; Tongzhou Maternal and Child Health Hospital of Beijing, Beijing 101100, China.
  • Chen L; Six Medical Center, Chinese PLA General Hospital, Beijing 100048, China.
  • Li H; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Medical School, University of Chinese Academy of Sciences, Beijing 100049, China; Beijing Institute for Stem Cell and Regenerative Medicine, Institute for Stem Cell a
  • Shi P; Annoroad Gene Technology, Beijing 100176, China.
  • Wang N; Annoroad Gene Technology, Beijing 100176, China.
  • Wei Z; Annoroad Gene Technology, Beijing 100176, China.
  • Wang YL; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Medical School, University of Chinese Academy of Sciences, Beijing 100049, China; Beijing Institute for Stem Cell and Regenerative Medicine, Institute for Stem Cell a
  • Jin L; Institute of Reproductive and Child Health, Peking University, National Health Commission Key Laboratory, Peking University, Beijing 100191, China; Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing 100191, China. Electronic address: jinlei@bjmu.edu.cn.
  • Du P; MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China. Electronic address: pengdu@pku.edu.cn.
  • Dong J; GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macau Joint Laboratory for Cell Fate Regulation and Diseases, Guangzhou National Laboratory, Guangzhou Medical University, Guangzhou 510799, China. Electronic address: dong_ji@gzlab.ac.cn.
  • Jiao J; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Medical School, University of Chinese Academy of Sciences, Beijing 100049, China; Beijing Institute for Stem Cell and Regenerative Medicine, Institute for Stem Cell a
Cell ; 186(26): 5892-5909.e22, 2023 12 21.
Article em En | MEDLINE | ID: mdl-38091994
Different functional regions of brain are fundamental for basic neurophysiological activities. However, the regional specification remains largely unexplored during human brain development. Here, by combining spatial transcriptomics (scStereo-seq) and scRNA-seq, we built a spatiotemporal developmental atlas of multiple human brain regions from 6-23 gestational weeks (GWs). We discovered that, around GW8, radial glia (RG) cells have displayed regional heterogeneity and specific spatial distribution. Interestingly, we found that the regional heterogeneity of RG subtypes contributed to the subsequent neuronal specification. Specifically, two diencephalon-specific subtypes gave rise to glutamatergic and GABAergic neurons, whereas subtypes in ventral midbrain were associated with the dopaminergic neurons. Similar GABAergic neuronal subtypes were shared between neocortex and diencephalon. Additionally, we revealed that cell-cell interactions between oligodendrocyte precursor cells and GABAergic neurons influenced and promoted neuronal development coupled with regional specification. Altogether, this study provides comprehensive insights into the regional specification in the developing human brain.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Encéfalo / Transcriptoma Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Encéfalo / Transcriptoma Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article