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Ex Vivo Surface Decoration of Phenylboronic Acid onto Natural Killer Cells for Sialic Acid-Mediated Versatile Cancer Cell Targeting.
Jangid, Ashok Kumar; Kim, Sungjun; Park, Hee Won; Kim, Hyun Jin; Kim, Kyobum.
Afiliação
  • Jangid AK; Department of Chemical & Biochemical Engineering, College of Engineering, Dongguk University, Seoul 04620, South Korea.
  • Kim S; Department of Chemical & Biochemical Engineering, College of Engineering, Dongguk University, Seoul 04620, South Korea.
  • Park HW; Department of Chemical & Biochemical Engineering, College of Engineering, Dongguk University, Seoul 04620, South Korea.
  • Kim HJ; Department of Biological Engineering, College of Engineering, Inha University, Incheon 22212, South Korea.
  • Kim K; Department of Chemical & Biochemical Engineering, College of Engineering, Dongguk University, Seoul 04620, South Korea.
Biomacromolecules ; 25(1): 222-237, 2024 01 08.
Article em En | MEDLINE | ID: mdl-38130077
ABSTRACT
Phenylboronic acid (PBA) has been highly acknowledged as a significant cancer recognition moiety in sialic acid-overexpressing cancer cells. In this investigation, lipid-mediated biomaterial integrated PBA molecules onto the surface of natural killer (NK) cells to make a receptor-mediated immune cell therapeutic module. Therefore, a 1,2-distearoyl-sn-glycero-3-phosphorylethanolamine (DSPE) lipid-conjugated di-PEG-PBA (DSPEPEG-di(PEG-PBA) biomaterial was synthesized. The DSPEPEG-di(PEG-PBA) biomaterial exhibited a high affinity for sialic acid (SA), confirmed by fluorescence spectroscopy at pH 6.5 and 7.4. DSPEPEG-di(PEG-PBA) was successfully anchored onto NK cell surfaces (PBA-NK), and this biomaterial maintains intrinsic properties such as viability, ligand availability (FasL & TRAIL), and cytokine secretion response to LPS. The anticancer efficacy of PBA-NK cells was evaluated against 2D cancer cells (MDA-MB-231, HepG2, and HCT-116) and 3D tumor spheroids of MDA-MB-231 cells. PBA-NK cells exhibited greatly enhanced anticancer effects against SA-overexpressing cancer cells. Thus, PBA-NK cells represent a new anticancer strategy for cancer immunotherapy.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácido N-Acetilneuramínico / Neoplasias Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácido N-Acetilneuramínico / Neoplasias Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article