Culture of Mouse Thymic Epithelial Cells in Serum-Free Medium.
Methods Mol Biol
; 2749: 1-6, 2024.
Article
em En
| MEDLINE
| ID: mdl-38133769
ABSTRACT
Primary cell culture systems are widely used as a valuable method for analyzing the biological functions of specific cells in vitro. Recently, various serum-free primary cell culture methods have been developed that do not involve the use of animal serums. Since the thymus is comprised of many cell types, such as thymocytes, thymic epithelial cells, macrophages, and fibroblasts, thymic epithelial cells must be isolated for their functional analysis in vitro. This chapter describes the detailed protocol for the selective primary culture of thymic epithelial cells using defined serum-free medium.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Timo
/
Células Epiteliais
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article