CRISPR/Cas9-Mediated Modification of PTP Expression.
Methods Mol Biol
; 2743: 43-56, 2024.
Article
em En
| MEDLINE
| ID: mdl-38147207
ABSTRACT
Alteration of protein tyrosine phosphatase (PTP) gene expression is a commonly used approach to experimentally analyze their function in the cell physiology of mammalian cells. Here, exemplified for receptor-type PTPRJ (Dep-1, CD148) and PPTRC (CD45), we provide the CRISPR/Cas9-mediated approaches for their inactivation and transcriptional activation using genome editing. These methods are generally applicable to any other protein of interest.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sistemas CRISPR-Cas
/
Edição de Genes
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article