An integrated plant glucose monitoring system based on microneedle-enabled electrochemical sensor.

ABSTRACT

Real-time monitoring of glucose concentration changes in plants and access to plant physiological information timely are of great significance to the development of precision agriculture. Here, we innovatively present an electrochemical sensing device that combines microneedle sensors and 3D printing technology to achieve real-time monitoring of glucose in plants in a minimally invasive manner. The device consists of two components the inner part features a highly efficient sensing interface based on platinum wire (MPt-Au-Nafion-GOx-Pu), while the outer part consists of polymer microneedles formed by 3D printing. Additionally, the polymer hollow microneedle features a slender tip diameter of only 300 µm, minimizing plant damage during the detection procedure. The device shows good detection performance, with a limit of detection (LOD) of 33.3 µM and a detection sensitivity of 17 nA/µM·cm2. It can detect glucose concentrations in the range of 100 µM to 100 mM, providing a unique solution for timely agronomic management of crops tool. By performing 12 h real-time monitoring and salt stress treat on tomato and aloe vera, the results verified the feasibility of integrated device applied to real-time glucose detection in plants.