Pathomolecular epidemiology, antimicrobial resistance, and virulence genes of Streptococcus dysgalactiae subsp. equisimilis isolates from slaughtered pigs in India.
J Appl Microbiol
; 135(1)2024 Jan 02.
Article
em En
| MEDLINE
| ID: mdl-38178631
ABSTRACT
AIMS:
We aimed to investigate the prevalence, pathology, and characterization of Streptococcus dysgalactiae subsp. equisimilis (SDSE) in slaughtered pigs of India. METHODS ANDRESULTS:
We collected 1254 morbid tissues (lungs-627 and spleen-627) and 627 heart-blood from 627 slaughtered pigs. The bacterial isolation, antibiogram, virulence gene profiling, and mouse pathogenicity testing were performed for the detection and characterization of SDSE. A total of 177 isolates (heart-blood-160 and tissues-17) were recovered from 627 slaughtered pigs with higher isolation rate in heart-blood (25.51%). The prevalence of SDSE was 11% in morbid tissues by polymerase chain reaction. Majority of isolates showed higher detection of streptolysin O, followed by streptokinase and extracellular phospholipase A virulence genes with higher degree of resistance to azithromycin, clindamycin, erythromycin, and penicillin antibiotics. Mouse pathogenicity testing confirmed virulence based on histopathological lesions and re-isolation of SDSE.CONCLUSIONS:
Our findings highlight the high prevalence of SDSE in slaughtered pigs. The presence of virulence genes and mouse pathogenicity testing confirm their pathogenic potential.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Infecções Estreptocócicas
/
Streptococcus
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Antibacterianos
Tipo de estudo:
Risk_factors_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article