Mechanistic Insights into the Adsorption of Monoclonal Antibodies at the Water/Vapor Interface.
Mol Pharm
; 21(2): 704-717, 2024 Feb 05.
Article
em En
| MEDLINE
| ID: mdl-38194618
ABSTRACT
Monoclonal antibodies (mAbs) are active components of therapeutic formulations that interact with the water-vapor interface during manufacturing, storage, and administration. Surface adsorption has been demonstrated to mediate antibody aggregation, which leads to a loss of therapeutic efficacy. Controlling mAb adsorption at interfaces requires a deep understanding of the microscopic processes that lead to adsorption and identification of the protein regions that drive mAb surface activity. Here, we report all-atom molecular dynamics (MD) simulations of the adsorption behavior of a full IgG1-type antibody at the water/vapor interface. We demonstrate that small local changes in the protein structure play a crucial role in promoting adsorption. Also, interfacial adsorption triggers structural changes in the antibody, potentially contributing to the further enhancement of surface activity. Moreover, we identify key amino acid sequences that determine the adsorption of antibodies at the water-air interface and outline strategies to control the surface activity of these important therapeutic proteins.
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Base de dados:
MEDLINE
Assunto principal:
Vapor
/
Anticorpos Monoclonais
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article