Bioinformatic Approach of B and T Cell Epitopes of PLD and CP40 Proteins of Corynebacterium pseudotuberculosis ovis Mexican Isolate 2J-L towards a Peptide-Based Vaccine.
Int J Mol Sci
; 25(1)2023 Dec 23.
Article
em En
| MEDLINE
| ID: mdl-38203441
ABSTRACT
Mapping B and T cell epitopes constitutes an important action for peptide vaccine design. PLD and CP40 virulence factors of Corynebacterium pseudotuberculosis biovar ovis, a causal agent of Caseous Lymphadenitis, have been evaluated in a murine model as good candidates for vaccine development. Therefore, the goal of this work was to in silico analyze B and T cell epitopes of the PLD and CP40 proteins of a Mexican isolate of Corynebacterium pseudotuberculosis ovis. The Immune Epitope Data Base and Resource website was employed to predict the linear and conformational B-cell, T CD4+, and T CD8+ epitopes of PLD and CP40 proteins of Corynebacterium pseudotuberculosis ovis Mexican strain 2J-L. Fifty B cell epitopes for PLD 2J-L and forty-seven for CP40 2J-L were estimated. In addition, T CD4+ and CD8+ cell epitopes were predicted for PLD 2J-L (MHC I16 epitopes, MHC II10 epitopes) and CP40 2J-L (MHC I 15 epitopes, MHC II 13 epitopes). This study provides epitopes, paying particular attention to sequences selected by different predictor programs and overlap sequences as B and T cell epitopes. PLD 2J-L and CP40 2J-L protein epitopes may aid in the design of a promising peptide-based vaccine against Caseous Lymphadenitis in Mexico.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Corynebacterium pseudotuberculosis
/
Infecções por Corynebacterium
/
Linfadenite
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País como assunto:
Mexico
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article