Bromine indirubin FLIM/PLIM sensors to measure oxygen in normoxic and hypoxic PDT conditions.
Photodiagnosis Photodyn Ther
; 45: 103964, 2024 Feb.
Article
em En
| MEDLINE
| ID: mdl-38218570
ABSTRACT
BACKGROUND:
The induction of phototoxicity during photodynamic therapy (PDT) is dependent on oxygen availability. For this reason, the development of sensors to measure oxygen and oxygen consumption is extremely important.APPROACH:
In this project we have used Fluorescence Lifetime imaging (FLIM) and Phosphorescence Lifetime Imaging/ delayed Fluorescence Lifetime Imaging (PLIM/dFLIM) to investigate the ability of bromine indirubin derivatives as oxygen sensors.RESULTS:
The oxygen sensitivity of bromine indirubins was detected through PLIM/dFLIM. Moreover, we have observed, by measuring nicotinamide adenine dinucleotide (NADH) FLIM, that bromine indirubin has a significant impact on cellular metabolism by shifting the SCC-4 Cells metabolism from oxidative phosphorylation (OXPHOS) to glycolysis.CONCLUSIONS:
In conclusion, this study successfully achieves its goals and provides important insights into the use of indirubin as a potential oxygen consumption sensor with the capability to identify and differentiate between normoxic and hypoxic regions within the cells.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Oxigênio
/
Fotoquimioterapia
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article