Negative role of filamentous bulking and its elimination in anammox process.

ABSTRACT

In this study, the filamentous bulking (FB) with moderate and excessive levels were demonstrated to induce anammox failure by inhibiting nitrogen (N) removal and biomass retention. The low external mass transfer resulted from high liquid-surface friction and low turbulence of filamentous surface was considered the "trigger" of anammox failure, which decreased flux of nitrogen flow toward granular surface and directly limited N-removal loading, which meanwhile exposed granules with N-scarcity environment and indirectly inhibited N-removal bio-activity. Low bio-activity performed poor extracellular polymeric substances secretion further destroyed bio-aggregation with low suface hydrophobicity, which acted as "accelerator" for granule disintegration and biomass washout, ultimatly leading to anammox failure. Fortunately, incresing hydraulic shear stress could eradicate FB's negative effects without inhibiting FB itself, which promoted re-granulation and N-remval restore by enhancing external mass transfer more than hydraulic detachment. Enhancing mechanical stirring with FB level was necessary to maintain stable operation of granular anammox system.