An end-to-end workflow for nondestructive 3D pathology.
Nat Protoc
; 19(4): 1122-1148, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38263522
ABSTRACT
Recent advances in 3D pathology offer the ability to image orders of magnitude more tissue than conventional pathology methods while also providing a volumetric context that is not achievable with 2D tissue sections, and all without requiring destructive tissue sectioning. Generating high-quality 3D pathology datasets on a consistent basis, however, is not trivial and requires careful attention to a series of details during tissue preparation, imaging and initial data processing, as well as iterative optimization of the entire process. Here, we provide an end-to-end procedure covering all aspects of a 3D pathology workflow (using light-sheet microscopy as an illustrative imaging platform) with sufficient detail to perform well-controlled preclinical and clinical studies. Although 3D pathology is compatible with diverse staining protocols and computationally generated color palettes for visual analysis, this protocol focuses on the use of a fluorescent analog of hematoxylin and eosin, which remains the most common stain used for gold-standard pathological reports. We present our guidelines for a broad range of end users (e.g., biologists, clinical researchers and engineers) in a simple format. The end-to-end workflow requires 3-6 d to complete, bearing in mind that data analysis may take longer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imageamento Tridimensional
/
Microscopia
Tipo de estudo:
Guideline
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article