Effect and mechanism of recombinant human fibroblast growth factor 18 on osteoporosis in OVX mice.
Climacteric
; 27(3): 305-313, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38275172
ABSTRACT
OBJECTIVES:
This study aimed to investigate the effect and the mechanism of recombinant human fibroblast growth factor 18 (rhFGF18) on postmenopausal osteoporosis.METHODS:
The effect of rhFGF18 on the proliferation and apoptosis of osteoblasts and the mechanism underlying such an effect was evaluated using an oxidative stress model of the MC3T3-E1 cell line. Furthermore, ovariectomy was performed on ICR mice to imitate estrogen-deficiency postmenopausal osteoporosis. Bone metabolism and bone morphological parameters in the ovariectomized (OVX) mice were evaluated.RESULTS:
The results obtained from the cell model showed that FGF18 promoted MC3T3-E1 cell proliferation by activating the extracellular signal-regulated kinase (ERK) and p38 instead of c-Jun N-terminal kinase (JNK). FGF18 also prevented cells from damage inflicted by oxidative stress via inhibition of apoptosis. After FGF18 administration, the expression level of anti-apoptotic protein Bcl-2 in the mice was upregulated, whereas those of the pro-apoptotic proteins Bax and caspase-3 were downregulated. Administering FGF18 also improved bone metabolism and bone morphological parameters in OVX mice.CONCLUSIONS:
FGF18 could effectively prevent bone loss in OVX mice by enhancing osteoblastogenesis and protecting osteoblasts from oxidative stress-induced apoptosis.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
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Proteínas Recombinantes
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Ovariectomia
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Osteoporose Pós-Menopausa
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Apoptose
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Estresse Oxidativo
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Proliferação de Células
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Modelos Animais de Doenças
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Fatores de Crescimento de Fibroblastos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article