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Biomarkers of Alzheimer's disease and neurodegeneration in dried blood spots-A new collection method for remote settings.
Huber, Hanna; Blennow, Kaj; Zetterberg, Henrik; Boada, Mercé; Jeromin, Andreas; Weninger, Haley; Nuñez-Llaves, Raul; Aguilera, Núria; Ramis, Maribel; Simrén, Joel; Nilsson, Johanna; Lantero-Rodriguez, Juan; Orellana, Adelina; García-Gutiérrez, Fernando; Morató, Xavier; Ashton, Nicholas J; Montoliu-Gaya, Laia.
Afiliação
  • Huber H; Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, the Shagreens Academy at the University of Gothenburg, Mölndal, Sweden.
  • Blennow K; Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, the Shagreens Academy at the University of Gothenburg, Mölndal, Sweden.
  • Zetterberg H; Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, Mölndal, Sweden.
  • Boada M; Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, the Shagreens Academy at the University of Gothenburg, Mölndal, Sweden.
  • Jeromin A; Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, Mölndal, Sweden.
  • Weninger H; Department of Neurodegenerative Disease, UCL Institute of Neurology, London, UK.
  • Nuñez-Llaves R; UK Dementia Research Institute, UCL, London, UK.
  • Aguilera N; Hong Kong Center for Neurodegenerative Diseases, Hong Kong, China.
  • Ramis M; Wisconsin Alzheimer's Disease Research Center, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA.
  • Simrén J; Networking Research Center on Neurodegenerative Diseases (CIBERNED), Instituto de Salud Carlos III, Madrid, Spain.
  • Nilsson J; Ace Alzheimer Center Barcelona, International University of Catalunya (UIC), Barcelona, Spain.
  • Lantero-Rodriguez J; ALZ Path Inc., Carlsbad, California, USA.
  • Orellana A; Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, the Shagreens Academy at the University of Gothenburg, Mölndal, Sweden.
  • García-Gutiérrez F; Ace Alzheimer Center Barcelona, International University of Catalunya (UIC), Barcelona, Spain.
  • Morató X; Ace Alzheimer Center Barcelona, International University of Catalunya (UIC), Barcelona, Spain.
  • Ashton NJ; Ace Alzheimer Center Barcelona, International University of Catalunya (UIC), Barcelona, Spain.
  • Montoliu-Gaya L; Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, the Shagreens Academy at the University of Gothenburg, Mölndal, Sweden.
Alzheimers Dement ; 20(4): 2340-2352, 2024 04.
Article em En | MEDLINE | ID: mdl-38284555
ABSTRACT

BACKGROUND:

We aimed to evaluate the precision of Alzheimer's disease (AD) and neurodegeneration biomarker measurements from venous dried plasma spots (DPSv enous) for the diagnosis and monitoring of neurodegenerative diseases in remote settings.

METHODS:

In a discovery (n = 154) and a validation cohort (n = 115), glial fibrillary acidic protein (GFAP); neurofilament light (NfL); amyloid beta (Aß) 40, Aß42; and phosphorylated tau (p-tau181 and p-tau217) were measured in paired DPSvenous and ethylenediaminetetraacetic acid plasma samples with single-molecule array. In the validation cohort, a subset of participants (n = 99) had cerebrospinal fluid (CSF) biomarkers.

RESULTS:

All DPSvenous and plasma analytes correlated significantly, except for Aß42. In the validation cohort, DPSvenous GFAP, NfL, p-tau181, and p-tau217 differed between CSF Aß-positive and -negative individuals and were associated with worsening cognition.

DISCUSSION:

Our data suggest that measuring blood biomarkers related to AD pathology and neurodegeneration from DPSvenous extends the utility of blood-based biomarkers to remote settings with simplified sampling conditions, storage, and logistics. HIGHLIGHTS A wide array of biomarkers related to Alzheimer's disease (AD) and neurodegeneration were detectable in dried plasma spots (DPSvenous). DPSvenous biomarkers correlated with standard procedures and cognitive status. DPSvenous biomarkers had a good diagnostic accuracy discriminating amyloid status. Our findings show the potential interchangeability of DPSvenous and plasma sampling. DPSvenous may facilitate remote and temperature-independent sampling for AD biomarker measurement. Innovative tools for blood biomarker sampling may help recognizing the earliest changes of AD.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doença de Alzheimer Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doença de Alzheimer Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article