Wavelength-Resolved Janus Biosensing Interface for Ratiometric Electrochemical Analysis.
Anal Chem
; 96(6): 2582-2589, 2024 02 13.
Article
em En
| MEDLINE
| ID: mdl-38294965
ABSTRACT
The Janus interface, comprising multiple functional heterointerfaces with contrasting functionalities within a single interface, has recently garnered widespread research interest. Herein, a Janus biosensing interface is obtained via wavelength-resolved laser illumination. Deoxyribonucleic acid bridges the electrochemical probe of methylene blue (MB) and plasmonic gold nanoparticles (AuNPs), achieving a sensitive detection performance. MB shows differential electrochemical signals under front (I532front) and back (I650back) laser illumination at 532 and 650 nm, respectively, owing to the selective wavelength-resolved effect. Thus, the presence of a wavelength-resolved laser enabled the design of a biosensing interface with Janus properties. The change in the distance between MB and AuNPs induced by aflatoxin B1 (AFB1) indicates that a sensitive response of the Janus biosensing interface can be achieved. A ratiometric strategy is introduced to describe the electrochemical signals of the I532front and I650back for improved robustness. The obtained linear range is 0.0005-50 ng mL-1, with a detection limit of 0.175 pg mL-1. Our study demonstrated that the wavelength-resolved Janus interface enables an electrochemical biosensor with excellent sensitivity. This finding provides an efficient approach for improving biosensor performance.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Aptâmeros de Nucleotídeos
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Nanopartículas Metálicas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article