Facial on-line enrichment of glycoproteins by capillary electrophoresis with boronate-functionalized poly(glycidyl methacrylate) microparticles coated column.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1234: 124013, 2024 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-38295722
ABSTRACT
A facial and rapid method for glycoproteins enrichment by capillary electrophoresis was developed. The 3-aminophenylboronic acid-functionalized poly(glycidyl methacrylate) microparticles (PGMA@APBA) were attached to the capillary inlet (length of â¼1.5 cm) by electrostatic self-assemble action to prepare a partially coated capillary column. The process is simple and reversible, allowing for easy renewal of the PGMA@APBA coating when its enrichment efficiency decreases. By utilizing the coated column, glycoproteins can be enriched within 2 min. The column exhibits a specific enrichment for glycoproteins and can be consecutively used for approximately 60 runs. The relative standard deviations (RSDs) of peak area of run-to-run (n = 5) and batch-to-batch (n = 3) were 1.5 % and 1.0%, respectively. The method was successfully applied to enrich glycoproteins from 1 × 1012-fold diluted real egg white sample, indicating its practical applicability.
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Base de dados:
MEDLINE
Assunto principal:
Ácidos Polimetacrílicos
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Eletroforese Capilar
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Compostos de Epóxi
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Metacrilatos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article