MEMS-based two-photon microscopy with Lissajous scanning and image reconstruction under a feed-forward control strategy.
Opt Express
; 32(2): 1421-1437, 2024 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-38297694
ABSTRACT
Two-photon microscopy (TPM) based on two-dimensional micro-electro-mechanical (MEMS) system mirrors shows promising applications in biomedicine and the life sciences. To improve the imaging quality and real-time performance of TPM, this paper proposes Lissajous scanning control and image reconstruction under a feed-forward control strategy, a dual-parameter alternating drive control algorithm and segmented phase synchronization mechanism, and pipe-lined fusion-mean filtering and median filtering to suppress image noise. A 10 fps frame rate (512 × 512 pixels), a 140â
µm × 140 µm field of view, and a 0.62 µm lateral resolution were achieved. The imaging capability of MEMS-based Lissajous scanning TPM was verified by ex vivo and in vivo biological tissue imaging.
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MEDLINE
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Ano de publicação:
2024
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Article