Protocol for immunophenotyping out-of-hospital cardiac arrest patients.
STAR Protoc
; 5(1): 102874, 2024 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-38310512
ABSTRACT
Immunophenotyping of out-of-hospital cardiac arrest (OHCA) patients is of increasing interest but has challenges. Here, we describe steps for the design of the clinical cohort, planning patient enrollment and sample collection, and ethical review of the study protocol. We detail procedures for blood sample collection and cryopreservation of peripheral blood mononuclear cells (PBMCs). We detail steps to modulate immune checkpoints in OHCA PBMC ex vivo. This protocol also has relevance for immunophenotyping other types of critical illness. For complete details on the use and execution of this protocol, please refer to Tamura et al. (2023).1.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Leucócitos Mononucleares
/
Parada Cardíaca Extra-Hospitalar
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article