Synthesis and Properties of RNA Modified with Cationic Amine Internucleoside Linkage.
ACS Chem Biol
; 19(2): 249-253, 2024 02 16.
Article
em En
| MEDLINE
| ID: mdl-38314716
ABSTRACT
Chemical modifications of RNA are important tools for the development of RNA therapeutics. The present study reports a novel RNA backbone modification that replaces the negatively charged phosphate with a positively charged amine linkage. Despite being thermally destabilizing in RNA duplexes, the amine linkage caused a relatively modest decrease of activity of a modified short interfering RNA (siRNA). At position 2 of the guide strand, the amine modification strongly enhanced the specificity of siRNA while causing an â¼5-fold drop of on-target activity. These results support the future development of amines as cationic RNA modifications and novel tools to modulate protein-RNA interactions.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA de Cadeia Dupla
/
Aminas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article