Intragenic Agrobacterium-mediated gene transfer mimics micro-translocations without foreign DNA.
Planta
; 259(3): 61, 2024 Feb 06.
Article
em En
| MEDLINE
| ID: mdl-38319406
ABSTRACT
MAIN CONCLUSION:
Agrobacterium-mediated transformation of Nicotiana tabacum, using an intragenic T-DNA region derived entirely from the N. tabacum genome, results in the equivalence of micro-translocations within genomes. Intragenic Agrobacterium-mediated gene transfer was achieved in Nicotiana tabacum using a T-DNA composed entirely of N. tabacum DNA, including T-DNA borders and the acetohydroxyacid synthase gene conferring resistance to sulfonylurea herbicides. Genomic analysis of a resulting plant, with single locus inheritance of herbicide resistance, identified a single insertion of the intragenic T-DNA on chromosome 5. The insertion event was composed of three N. tabacum DNA fragments from other chromosomes, as assembled on the T-DNA vector. This validates that intragenic transformation of plants can mimic micro-translocations within genomes, with the absence of foreign DNA.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Acetolactato Sintase
/
Rearranjo Gênico
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article