Comparison of different concentrations of chlorhexidine-iodophor composite solution on human skin fibroblasts.
J Wound Care
; 33(Sup2a): xxxii-xl, 2024 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-38324419
ABSTRACT
OBJECTIVE:
Chlorhexidine-iodophor (CHX-IP) composite solution is a polymer of chlorhexidine and iodophor produced with new technology, for use in diabetic foot infection. However, the effect of CHX-IP on the growth activity of fibroblasts remains unknown, thus the effects of different concentrations of CHX-IP composite solution on the viability and micromorphology of human skin fibroblasts were studied in vitro cell culture in this study.METHOD:
A cell viability assay was applied to calculate cell viability and an inverted fluorescence microscope was used to observe cell morphology over five days.RESULTS:
The results showed that the toxic effect of CHX-IP on fibroblasts was solution concentration-dependent and decreased over time. When the concentration of CHX-IP was 5.0mg/ml, 2.5mg/ml, 0.625mg/ml, 0.15625mg/ml, 0.078125mg/ml or 0mg/ml, the difference of optical density (OD) value on different days was statistically significant (p<0.05). There were statistically significant differences in the OD value of fibroblasts among different concentrations of CHX-IP on day 2 (F=4.809, p=0.004); day 3 (F=21.508, p<0.001); day 4 (F=63.952, p<0.001); and day 5 (F=160.407, p<0.001). In addition, a concentration of 5.0mg/ml CHX-IP resulted in a fibroblastic viability rate of 0% on day 4, when CHX-IP was diluted to 2.5mg/ml or 1.25 mg/ml, fibroblastic viability rate decreased to 0% day 5. However, when the CHX-IP was diluted to 0.15625mg/ml or 0.078125mg/ml, the fibroblastic cell viability rate increased slightly on day 5. The morphology of cells observed under microscope indirectly supported this result.CONCLUSION:
The findings of this study showed that the toxic effect of CHX-IP on fibroblasts was solution concentration-dependent and decreased over time.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Clorexidina
/
Anti-Infecciosos Locais
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article