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Serine protease inhibitor from the muscle larval Trichinella spiralis ameliorates non-alcoholic fatty liver disease in mice via anti-inflammatory properties and gut-liver crosstalk.
Tong, Mingwei; Yang, Xiaodan; Qiao, Yuyu; Liu, Ge; Ge, Huihui; Huang, Guangrong; Wang, Yanhong; Yang, Yong; Fan, Weiping.
Afiliação
  • Tong M; School of Basic Medical Sciences, Shanxi Medical University, Jinzhong 030619, China; Key Laboratory of Cellular Physiology (Shanxi Medical University), Ministry of Education, and Shanxi Key Laboratory of Cellular Physiology, Taiyuan 030001, China. Electronic address: tmwesse@126.com.
  • Yang X; School of Basic Medical Sciences, Shanxi Medical University, Jinzhong 030619, China.
  • Qiao Y; School of Basic Medical Sciences, Shanxi Medical University, Jinzhong 030619, China.
  • Liu G; School of Basic Medical Sciences, Shanxi Medical University, Jinzhong 030619, China.
  • Ge H; School of Basic Medical Sciences, Shanxi Medical University, Jinzhong 030619, China.
  • Huang G; School of Basic Medical Sciences, Shanxi Medical University, Jinzhong 030619, China.
  • Wang Y; School of Basic Medical Sciences, Shanxi Medical University, Jinzhong 030619, China; Key Laboratory of Cellular Physiology (Shanxi Medical University), Ministry of Education, and Shanxi Key Laboratory of Cellular Physiology, Taiyuan 030001, China.
  • Yang Y; School of Basic Medical Sciences, Shanxi Medical University, Jinzhong 030619, China; Key Laboratory of Cellular Physiology (Shanxi Medical University), Ministry of Education, and Shanxi Key Laboratory of Cellular Physiology, Taiyuan 030001, China. Electronic address: yongyang811128@163.com.
  • Fan W; School of Basic Medical Sciences, Shanxi Medical University, Jinzhong 030619, China; Key Laboratory of Cellular Physiology (Shanxi Medical University), Ministry of Education, and Shanxi Key Laboratory of Cellular Physiology, Taiyuan 030001, China. Electronic address: fanweiping26418@126.com.
Biomed Pharmacother ; 172: 116223, 2024 Mar.
Article em En | MEDLINE | ID: mdl-38325266
ABSTRACT
Trichinella spiralis is recognized for its ability to regulate host immune responses. The serine protease inhibitor of T. spiralis (Ts-SPI) participates in T. spiralis-mediated immunoregulatory effects. Studies have shown that helminth therapy exhibits therapeutic effects on metabolic diseases. In addition, we previously found that T. spiralis-derived crude antigens could alleviate diet-induced obesity. Thus, Ts-SPI was hypothesized to alleviate non-alcoholic fatty liver disease (NAFLD). Herein, recombinant Ts-SPI (rTs-SPI) was prepared from the muscle larvae T. spiralis. The relative molecular mass of rTs-SPI was approximately 35,000 Da, and western blot analysis indicated good immunoreactivity. rTs-SPI ameliorated hepatic steatosis, inflammation, and pyroptosis in NAFLD mice, which validated the hypothesis. rTs-SPI also reduced macrophage infiltration, significantly expanded Foxp3+ Treg population, and inactivated TLR4/NF-κB/NLRP3 signaling in the liver. Furthermore, rTs-SPI treatment significantly shifted the gut microbiome structure, with a remarkable increase in beneficial bacteria and reduction in harmful bacteria to improve gut barrier integrity. Finally, Abx-treated mice and FMT confirmed that gut-liver crosstalk contributed to NAFLD improvement after rTs-SPI treatment. Taken together, Taken together, these findings suggest that rTs-SPI exerts therapeutic effects in NAFLD via anti-inflammatory activity and gut-liver crosstalk.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Serpinas / Trichinella spiralis / Hepatopatia Gordurosa não Alcoólica Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Serpinas / Trichinella spiralis / Hepatopatia Gordurosa não Alcoólica Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article